簡要描述：Definitive Endoderm Differentiation Kit 定向分化獲取內胚層細胞試劑盒用于定向分化hiPS獲取定型內胚層細胞（DE）。包含所有必需的培養基和coating材料。還包括新一代的Cellartis DEF-CS 100 Culture System用于起始hiPS的維持培養用。獲得的DE細胞，可以作為起始材料進行定向分化至內胚層來源的細胞類型（如肝細胞，胰腺細胞）

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Cellartis定向分化獲取內胚層細胞試劑盒

產品英文名稱：Cellartis Definitive Endoderm Differentiation Kit

產品中文名稱：Cellartis定向分化獲取內胚層細胞試劑盒

用于定向分化hiPS獲取內胚層細胞

The Cellartis Definitive Endoderm Differentiation Kit用于定向分化hiPS獲取definitive endoderm (DE) cells。本產品包含所有必需的培養基和coating材料。本產品還包括新一代的Cellartis DEF-CS 100 Culture System用于起始hiPS的維持培養用。使用本產品獲得的DE細胞，可以作為起始材料進行定向分化至內胚層來源的細胞類型(如肝細胞，胰腺細胞)。Cellartis也提供分化DE細胞獲取肝細胞的試劑盒（Cellartis Hepatocyte Differentiation Kit (Cat. No. Y30050)；Cellartis也提供誘導分化hiPS獲取肝細胞的試劑盒（Cellartis iPS Cell to Hepatocyte Differentiation System (Cat. No. Y30055)。

The Cellartis with DEF-CS Culture System contains a complete media set and a ready-to-use coating for the differentiation of human induced pluripotent stem (hiPS) cells into definitive endoderm (DE) cells in monolayer culture. The kit includes the Cellartis DEF-CS 100 Culture System for the maintenance of undifferentiated hiPS cells prior to DE cell differentiation. The DE cells obtained with this differentiation kit can be used for further differentiation into cells of endodermal origin, such as hepatocytes and pancreatic endoderm cells. For further differentiation of DE cells into hepatocytes, use the Cellartis Hepatocyte Differentiation Kit (Cat. No. Y30050); alternay, start directly from iPS cells using the complete Cellartis iPS Cell to Hepatocyte Differentiation System (Cat. No. Y30055).

產品特點:

有效的分化 - 產生> 80％的SOX17陽性細胞

強大的培養方法 - 來自超過25種不同hiPS細胞系成功分化為定形內胚層（DE）細胞

完整的試劑盒 - 包括培養基和即用型包被，將hiPS細胞分化為DE細胞的實驗方案為期七天，具備可重復性

起始為均質的hiPS細胞群 - 試劑盒包括Cellartis DEF-CS 100培養系統以在DE細胞分化開始之前維持細胞的未分化狀態

Efficient differentiation—generates >80% SOX17-positive cells

Robust protocol—successful definitive endoderm (DE) cell generation from more than 25 different hiPS cell lines

Complete kit—media and ready-to-use coating for a seven-day protocol to reproducibly differentiate hiPS cells into DE cells

Start with a homogeneous population of hiPS cells—kit includes the Cellartis DEF-CS 100 Culture System to maintain undifferentiated cells prior to the start of DE cell differentiation

組份：iPS細胞培養完整試劑盒+分化內胚層試劑盒

Cellartis with DEF-CS Culture System (Cat. No. Y30035):

Cellartis DEF-CS 100 Culture System (Cat. No. Y30020; not sold separay. A larger 500 ml version of this product is sold as Cat. No. Y30010.)

? 100 ml DEF-CS 100 Basal Medium

? 800 µl DEF-CS COAT-1 (for 100 ml)

? 300 µl DEF-CS GF-1 (for 100 ml)

? 100 µl DEF-CS GF-2 (for 100 ml)

? 40 µl DEF-CS GF-3 (for 100 ml)

Cellartis (Cat. No. Y30030; not sold separay)

? 1 tube Definitive Endoderm Differentiation Coating (10 ml)

? 1 bottle Definitive Endoderm Differentiation Day 0 (18 ml)

? 1 bottle Definitive Endoderm Differentiation Day 1 (18 ml)

? 1 bottle Definitive Endoderm Differentiation Day 2 (18 ml)

? 1 bottle Definitive Endoderm Differentiation Day 3 (25 ml)

? 1 bottle Definitive Endoderm Differentiation Day 4 (47 ml)

? 1 bottle Definitive Endoderm Differentiation Day 6 (47 ml)

自備試劑：（可選）

人多能干細胞

PBS Dulbecco's含有Ca2 +和Mg2 +（D-PBS + / +）

PBS Dulbecco's不含Ca 2+和Mg 2+（D-PBS - / - ）

TrypLE選擇酶（1X），不含酚紅

保存：Cellartis DEF-CS 100 Culture System (Y30020)：DEF-CS 100 Basal Medium ，COAT-1   2–8°C；GF-1, GF-2, and GF-3  –20°C

Cellartis (Y30030)：–20°C

產品性能：

mRNA expression profiles of definitive endoderm cells being generated from two hiPS cell lines (ChiPSC18 and P11025) using the Cellartis with DEF-CS Culture Medium. These graphs track the relative temporal mRNA expression levels of various markers during differentiation from hiPS cells (starting on Day 0) into DE cells (by Day 7), as measured by RT-qPCR.

相關產品:

Cellartis Human ES Cell Line 121 (SA121) Kit (Cat No. Y00025)

Cellartis Human ES Cell Line 167 (SA167) Kit (Cat. No. Y00065)

Cellartis Human ES Cell Line 181 (SA181) Kit (Cat. No. Y00105)

Cellartis Human ES Cell Line 461 (SA461) Kit (Cat. No. Y00145)

Cellartis Human iPS Cell Line 7 (ChiPSC7) Kit (Cat. No. Y00275)

Cellartis Human iPS Cell Line 12 (ChiPSC12) Kit (Cat. No. Y00285)

Cellartis Human iPS Cell Line 18 (ChiPSC18) Kit (Cat. No. Y00305)

Cellartis Human iPS Cell Line 22 (ChiPSC22) Kit (Cat. No. Y00325)

Cellartis® Hepatocyte Differentiation Kit (Cat. No. Y30050)

Cellartis® iPS Cell to Hepatocyte Differentiation System (Cat. No. Y30055)

肝細胞培養完整解決方案

人iPS誘導分化成肝細胞全過程的完整試劑盒：Y30055（新產品）

人iPS來源肝細胞株及*培養基Y10133/Y10134/Y10135（新產品）

人原代肝臟細胞培養基 Y20020（新產品）

http://www.chem17.com/st321032/article_1496885.html

華雅再生醫學旗艦公司：紅榮微再（上海）生物工程技術有限公司主營細胞治療和精準醫療等產品，2018年與TAKARA繼續合作，授權代理（上海）其旗下Cellartis干細胞研究制品和Rubicon單細胞文庫構建試劑。Cellartis公司位于瑞典，擁有iPS細胞等干細胞分化為肝細胞及臟器細胞的分化誘導技術和ES細胞，ips細胞，分化細胞等干細胞相關產品。

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